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目的 研究蛋白激酶C(protein kinase C,PKC)信息通道在阻塞性黄疸机体发病机理中的作用。 方法 从51 例阻塞性黄疸患者和16 例正常对照者的外周静脉血中分离和纯化淋巴细胞胞浆及胞膜部分蛋白激酶C,然后采用同位素γ32PATP催化活性测定法检测它们的活性。 结果 (1)阻塞性黄疸患者外周血淋巴细胞蛋白激酶C的总活性较正常健康人明显增强( P< 0-01) ,且胞膜部分蛋白激酶C活性占总活性的百分值较正常组也明显升高( P< 0-05)。(2)阻塞性黄疸患者淋巴细胞蛋白激酶C的活性与血清黄疸的程度(TBIL) 显著正相关(r= 0-67,P< 0-01),与血清可溶性白细胞介素2 受体(soluteinterleukin2,sIL2R) 浓度显著正相关(r= 0-58 ,P< 0-01)。 结论 蛋白激酶C信息通道的激活与黄疸程度有关,且可能参与了阻塞性黄疸时T淋巴细胞的活化,对阻塞性黄疸时免疫调节及病情评估可能有重要意义
Objective To study the role of protein kinase C (PKC) signaling pathway in the pathogenesis of obstructive jaundice. Methods From 51 patients with obstructive jaundice and 16 normal controls in peripheral venous blood of lymphocytes isolated and purified cytoplasm and plasma membrane protein kinase C, and then using isotope γ 32P ATP catalytic activity assay to detect their activity . Results (1) The total activity of protein kinase C in peripheral blood lymphocytes in patients with obstructive jaundice was significantly higher than that in healthy controls (P <0-01), and the percentages of protein kinase C Also significantly higher (P <0-05). (2) The activity of lymphocyte protein kinase C in patients with obstructive jaundice was significantly and positively correlated with the level of serum jaundice (TBIL) (r = 0-67, P <0-01), but not with serum soluble interleukin2 (Soluteinterleukin 2, sIL 2R) concentration was significantly correlated (r = 0-58, P <0-01). Conclusion The activation of protein kinase C channel is related to the degree of jaundice, and may be involved in the activation of T lymphocytes in obstructive jaundice. It may play an important role in immune regulation and assessment of disease in obstructive jaundice