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目的分析家系成员EB病毒VCA-IgA抗体检测结果。方法对经县级及以上医院确诊的鼻咽癌先证者及其血缘父母进行调查和填写调查表,并采集外周静脉血8 mL,其中抗凝血5 mL分离、冻存,3 mL非抗凝血进行血清EB病毒VCA-IgA抗体滴度;对照组采用广西境内高发区自然人群。结果共收集鼻咽癌核心家系254例,建立家系成员资料数据库,并分析家系成员VCA-IgA抗体检测结果,其中先证者父母EB-VCA-IgA抗体阳性率显著高于对照组(P<0.01)。结论建立规范的鼻咽癌核心家系数据库为今后进一步利用分子遗传学方法来研究鼻咽癌的病因打下基础。
Objective To analyze the results of detection of Epstein-Barr virus VCA-IgA antibody in family members. Methods A survey of NPC probands and their relatives whose parents were diagnosed at county level and above was conducted and questionnaires were filled in. Eight mL of peripheral venous blood was collected, in which 5 mL of anticoagulated blood was isolated, frozen, and 3 mL of non-resistant Coagulation serum Epstein-Barr virus VCA-IgA antibody titers; control group using high-incidence areas in Guangxi natural population. Results A total of 254 nuclear pedigrees of NPC were collected and the pedigree database was established. The results of VCA-IgA antibody test in family members were analyzed. The positive rate of EB-VCA-IgA antibody in probands was significantly higher than that in control group (P <0.01) ). Conclusion The establishment of a standardized nuclear pedigree database for nasopharyngeal carcinoma lay the foundation for the further study of the etiology of nasopharyngeal carcinoma using molecular genetics.