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目的:以延胡索乙素和原阿片碱对照品为参照,建立延胡索真伪品的指纹图谱,提供一种鉴别延胡索与夏天无药材的质控方法。方法:采用反相高效液相色谱法,色谱柱为Kromasil-C_(18)柱(250mm×4.6 mm,5μm),流动相A:乙腈、B:0.1%磷酸溶液(三乙胺调pH 6.5),线性梯度洗脱,检测波长为280nm。结果:不同来源的正品延胡索HPLC指纹图谱十分相似,而与易混品相比则显示出完全不同的指纹图谱特征。结论:方法简便,准确,可用于延胡索与夏天无药材的鉴别依据。
OBJECTIVE: To establish a fingerprint of authentic Corydalis yanhusuo with reference to tetrahydropalmatine and original opioid reference substance, and provide a quality control method for identifying Corydalis yanhusuo and summer without medicinal materials. METHODS: A reversed-phase high performance liquid chromatographic method was used. The column was a Kromasil-C18 column (250 mm × 4.6 mm, 5 μm). Mobile phase A: acetonitrile, B: 0.1% phosphoric acid solution (triethylamine, pH 6.5) Linear gradient elution with a detection wavelength of 280 nm. RESULTS: HPLC fingerprints of authentic Corydalis from different sources were quite similar, but they showed completely different fingerprint features compared to the easily mixable products. Conclusion: The method is simple, accurate, and can be used to identify the basis of identification of Corydalis yanhusuo and no medicinal materials in summer.