Background Laparoendoscopic single-site surgery (LESS) approaches have been reported for treating various kidney and pelvic procedures,and are feasible and effective in selected patients.In this study,we aimed to present the initial experience and evaluate the efficacy of laparoscopic radical prostatectomy performed through a single incision using a multichannel port.Methods Between July 2010 and April 2011,six patients diagnosed with early stage prostate cancer underwent LESS radical prostatectomy (RP) in our institute.A multichannel port was inserted transperitoneally through a 2-cm umbilical incision.Specially articulating and flexible laparoscopic were used.Some technical tricks and points were applied during the operation to overcome the drawbacks and reduce the difficulties of this approach.Two continuous urethrovesical sutures in both sides were performed to complete both lateral aspects of anastomosis.The two ends of the suture threads were fixed by double Lapro-Clips,instead of the difficult knot-tying.Results Total operative time was (265±43) minutes.Mean blood loss was (230±65) ml.All cases were completed successfully,without conversion to open surgery or adding additional abdomen ports.No patient required a blood transfusion and no intraoperative complications occurred.The Foley catheter was removed at the 14th day (range 12th-16th) after surgery.At the 12th week of follow-up,all patients had an undetectable prostate-specific antigen level.Two patients used 2 or 1 pad for continence daily; other patients had achieved good continence.Conclusion In selected cases,LESS-RP is feasible and effective; these technic points and the flexible-articulating instruments are helpful to reduce the operation difficulties.