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目的 检测 Ｓ Ｖ４０ 早期区域基因编码产物大 Ｔ 抗原（ Ｔａｇ）在人脑肿瘤中的表达，探讨 Ｓ Ｖ４０ 与人脑肿瘤发生的病因学关系。方法 采用免疫共沉淀、银染色及 Ｗｅｓｔｅｒｎ印迹检测 ６５ 例人脑肿瘤组织、８ 例正常人脑组织及 ２ 株人脑胶质瘤细胞系中 Ｔａｇ 的表达。结果 Ｔａｇ 在 ８ 例室管膜瘤、２ 例脉络丛乳头状瘤及 ２ 株人脑胶质瘤细胞系中全部表达；垂体腺瘤 Ｔａｇ 阳性率为 ９０％ （９／１０），星形胶质细胞瘤７３％ （１１／１５），脑 膜瘤 ７０％ （７／１０），多形性 胶质母细胞 瘤５０％ （４／８），髓母细胞瘤 ３３％ （２／６）；５ 例少枝胶质细胞瘤、１例松果体瘤及 ８ 例正常人脑组织无 Ｔａｇ 表达。结论 Ｓ Ｖ４０感染与人脑肿瘤的发生有一定关系。
Objective To detect the expression of large T antigen (S Tag) encoded by early gene of S V40 in human brain tumor and to explore the etiological relationship between S V40 and human brain tumor. Methods Immuno-coprecipitation, silver staining and Western blotting were used to detect the expression of Tag in 65 human brain tumor tissues, 8 normal human brain tissues and 2 human glioma cell lines. Results Tag was expressed in 8 cases of ependymoma, 2 cases of choroid plexus papilloma and 2 human glioma cell lines. The positive rate of Tag was 90% (9/10) in pituitary adenomas, 73% (11/15), 70% (7/10) of meningioma, 50% (4/8) of glioblastoma and 33% (2/6) of medulloblastoma in 5 cases Juvenile glioblastoma, 1 case of pineal tumor and 8 cases of normal human brain tissue without Tag expression. Conclusion S V40 infection has a certain relationship with the occurrence of human brain tumors.