赤霉素（ｇｉｂｂｅｒｅｌｌｉｎｓ）是植物生长发育过程中一类重要的调节激素，在水稻不育系上喷施合适浓度的ＧＡ３（赤霉素的一种）可以克服其包颈现象．运用反转录和聚合酶链式反应（ＰＣＲ）建立了一套旨在分离差异表达。ＤＮＡ的差异展示方法．将赤霉素ＧＡ３处理后的苗期水稻分别制备总ｍＲＮＡ，反转录ＰＣＲ后在测序胶上比较各处理间基因表达的差异，发现其中两个基因的表达量随ＧＡ３浓度的增加而增强，另有一个基因的表达则随ＧＡ３浓度的增加而减弱．已从测序胶中回收前两个表达增强的基因的。ＤＮＡ片段并重新扩增，拟用作探针从水稻基因组文库中分离这两个受ＧＡ３调控的基因． Gibberellins is an important regulator of plant growth and development. The spraying of a suitable concentration of GA3 (a kind of gibberellin) on male sterile lines of rice can overcome its necking phenomenon. Using reverse transcription and polymerase chain reaction (PCR) to establish a set of aims to separate the differential expression. Differences in DNA display method. GA3-treated rice seedlings were prepared respectively total mRNA, reverse transcription PCR comparison of the gene expression differences between the various treatments and found that the expression of two genes increased with the GA3 concentration increased, Another gene expression decreased with increasing GA3 concentration. The first two genes with enhanced expression have been recovered from the sequencing gel. DNA fragments and re-amplified, to be used as a probe to isolate these two GA3-regulated genes from the rice genomic library.