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对在田间非隔离条件下连续种植的３ 个品种脱毒薯，用血清学ＮＣＭ－ ＥＬＩＳＡ 方法检测甘薯羽状斑驳病毒（ＳＰＦＭＶ） ，调查侵染率和反应强度，第１ 年就出现了较高侵染率，以后几乎每年增加１ 倍，第３ 年可接近对照水平。阳性强度也逐年增加，相关分析结果表明，病毒阳性增长率与鲜薯增产率呈极显著负相关。该结果从理论上证实了脱毒种薯使用年限为２ ～３ａ 。
In the field of non-isolation under continuous cultivation of three varieties of drug-free potato, using the method of serological NCM-ELISA detection of sweet potato feather mottle virus (SPFMV), investigation of infection rate and response intensity, the first year appeared higher Infection rates, doubling almost every year thereafter, approaching the control level in the third year. The positive intensity also increased year by year. The correlation analysis showed that the positive rate of virus growth was significantly negatively correlated with the yield of fresh potato. The results from the theoretical proof of virus-free seed pot life of 2 ~ 3a.