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作者介绍了一种特异的、有效的融合方法以获得分泌抗脱脂蛋白-A1单克隆抗体的杂交瘤。即在体外免疫之后,对免疫脾细胞作部分提纯。这种方法可大大提高融合率和分泌抗体的杂交瘤阳性率。
The authors describe a specific and efficient fusion method to obtain hybridomas that secrete anti-apolipoprotein-A1 monoclonal antibodies. That is, after immunization in vitro, the immune spleen cells were partially purified. This method can greatly improve the fusion rate and the positive rate of antibody secreting hybridomas.