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目的建立一种非标记、多指标的登革热快速检测方法,应用于登革热疑似病例的血清样本的快速检测。方法采用表面引发聚合反应技术构建基于寡聚乙二醇高分子的抗蛋白质非特异性吸附表面,以抗登革热IgM、IgG和抗登革热NS1抗体分别作为捕获探针,建立登革热的多指标微阵列芯片。采用表面等离子共振检测技术的技术手段,在血清中检测登革热特异性抗体。结果该技术方法对262份登革热疑似病例血清进行检测,阳性检出率为6.9%(18/262),高于PCR 2.3%(6/262)和ELISA 5.3%(14/262),其中PCR阳性的6份血清样本均检测到NS1标记物,而14份ELISA阳性样本中,检测出其中12份,两者的检测符合率为85.7%。结论初步构建一种登革热综合性蛋白质芯片,结合SPR技术可并行检测多个项目,可为登革热的快速检测提供一个有力的技术平台。
OBJECTIVE: To establish a rapid, non-labeled and multiplex dengue detection method for the rapid detection of suspected dengue samples. Methods Surface-initiated polymerization was used to construct non-specific surface anti-protein based on oligomeric polyethylene glycol. Anti-dengue IgM, IgG and anti-dengue NS1 antibodies were used as capture probes to establish dengue multi-index microarray. Using surface plasmon resonance detection technology, detection of dengue specific antibodies in serum. Results The method was used to detect 262 cases of suspected cases of dengue. The positive detection rate was 6.9% (18/262), higher than 2.3% (6/262) in PCR and 5.3% (14/262) in ELISA. PCR positive Of the 6 serum samples were detected NS1 markers, and 14 ELISA positive samples, 12 were detected, the detection coincidence rate of both 85.7%. Conclusion The preliminary construction of a dengue integrated protein chip, combined with SPR technology can detect multiple projects in parallel, which can provide a powerful technical platform for rapid detection of dengue fever.