随着生活节奏的加快和生活质量的提高,大量的日用产品及新型化妆品涌入我们的生活,对这些新产品进行毒理学安全性评价是相关部门制定卫生标准、进行管理的依据和基础。急性毒性实验是评价化妆品原料毒性的第一步,可对其进行毒性分级,也为其他毒性试验的剂量设计做重要参考。传统的急性毒性半数致死剂量(LD)测定方法有寇氏法、霍恩氏法、概率 With the accelerated pace of life and the improvement of quality of life, a large number of daily products and new cosmetics are pouring into our lives. The toxicological safety evaluation of these new products is the basis and foundation for the relevant departments to formulate health standards and management. Acute toxicity tests are the first step in assessing the toxicity of cosmetic raw materials, allowing them to be graded for toxicity, as well as an important reference for dose design for other toxicity tests. The traditional method of determination of acute lethal LD50 (Kodak’s method, Horn’s method, probability