论文部分内容阅读
目的:观察2-(2-苯并呋喃基)-2-咪唑啉[2-(2-benzofu-ranyl)-2-imidazoline,2-BFI]对局灶性脑缺血大鼠神经神经功能恢复和缺血半暗带区新生血管形成的影响。方法:制备SD大鼠大脑中动脉阻塞(MCAO)模型,随机分为假手术组、2-BFI组和对照组,通过改良神经功能缺损评分(modified Neurological Severity Scores,mNSS)、TTC染色、免疫组化学检测脑内vWF的表达、异硫氰酸荧光素右旋糖酐(FITC-dextran)法行脑微血管灌注成像等研究方法研究2-BFI对神经功能、脑缺血梗死区的体积、内皮细胞及血管新生影响。结果:①2-BFI组的神经功能缺损评分(NSS)有明显改善(P<0.05);②2-BFI组脑梗死体积百分比小于对照组(P<0.05);③2-BFI组阳性细胞数明显高于对照组(P<0.05);④2-BFI组的脑内缺血半暗带区域毛细血管分布较为密集且充盈状态良好,微血管显影面积明显高于对照组(P<0.05)。结论:2-BFI能促进脑缺血大鼠神经功能恢复,促进脑内缺血半暗带区域血管新生,增加微血管血流灌注。
OBJECTIVE: To observe the effects of 2- (2-benzofu-ranyl) -2-imidazoline (2-BFI) on neurological function in rats with focal cerebral ischemia And ischemic penumbra area neovascularization. Methods: The middle cerebral artery occlusion (MCAO) model of SD rats was established and randomly divided into sham operation group, 2-BFI group and control group. The modified Neurological Severity Scores (mNSS), TTC staining, The effect of 2-BFI on the neurological function, the volume of ischemic infarction area, endothelial cells and angiogenesis was studied by chemical detection of vWF expression in the brain and FITC-dextran perfusion imaging. influences. Results: ① The neurological deficit score (NSS) in 2-BFI group was significantly improved (P <0.05); ② The percentage of cerebral infarction volume in 2-BFI group was smaller than that in control group (P <0.05); ③The number of positive cells in 2-BFI group was significantly higher than that in control group (P <0.05). ④ In the 2-BFI group, the capillaries in the penumbra area of the cerebral ischemic area were densely packed and the state of filling was good. The area of microvascular development was significantly higher than that of the control group (P <0.05). Conclusions: 2-BFI can promote neurological function recovery, promote angiogenesis in ischemic penumbra area and increase microvascular perfusion in cerebral ischemia rats.