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铁是植物必需的微量元素.除了铁的吸收机理Ⅰ和机理Ⅱ外,膜泡运输过程参与铁吸收及铁稳态的维持也有所报道.报道了一个新的膜泡(vesicle)相关基因OsSEC27P,并对其功能进行了分析.基因芯片和实时定量PCR证明,OsSEC27P受缺铁条件诱导上调表达.在转基因烟草悬浮细胞BY-2中表达的OsSEC27P-GFP融合蛋白和FM4-64共定位,证明OsSEC27P蛋白主要集中于细胞内的膜泡中.OsSEC27P过量表达的转基因烟草液体培养基的pH明显下降,通过离子选择性电极扫描技术(SIET),证明是小苗根部缺铁响应的质子分泌显著加强所致.综上认为,膜泡相关蛋白OsSEC27P在缺铁条件下,对增强根的H+释放起着重要的作用.
Iron is a necessary trace element for plants.In addition to iron absorption mechanismⅠand mechanismⅡ, vesicular transport involved in iron absorption and maintenance of iron homeostasis has also been reported.A new vesicle-associated gene OsSEC27P, And its function was analyzed.Gene microarray and real-time quantitative PCR showed that OsSEC27P was up-regulated by iron deficiency condition.The co-localization of OsSEC27P-GFP fusion protein and FM4-64 expressed in BY-2 transgenic tobacco showed that OsSEC27P The protein mainly concentrated in the intracellular vesicles.The pH of transgenic tobacco liquid medium with overexpression of OsSEC27P decreased significantly, which was demonstrated by the ion selective electrode scanning (SIET) In conclusion, the membrane-associated protein OsSEC27P plays an important role in enhancing root H + release under iron deficiency.