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目的:研究云木香挥发油、4种水提液萃取物对豚鼠离体肠平滑肌及小鼠胃排空和小肠推进功能的影响。方法:采用BL-420F生物机能实验系统、改良的酚红含量测定法,观察挥发油、4种水提液萃取物对豚鼠离体肠平滑肌及小鼠胃排空和小肠推进功能的作用情况。结果:云木香挥发油(4.0×10-2g生药/ml)、水提液萃取物(1.0×10-2g生药/ml)能够显著降低豚鼠正常状态下;挥发油(2.0×10-2g生药/ml)、水提液萃取物(1.5×10-2g生药/ml)能够显著降低Ach所致痉挛状态下豚鼠离体肠平滑肌的平均振幅;挥发油(2.4×10-2g生药/ml)、水提液萃取物(2.4×10-2g生药/ml)能够显著降低正常小鼠胃酚红排空率,显著提高阿托品所致抑制状态下的小鼠胃酚红排空率,并显著降低新斯的明所致亢进状态下的小鼠胃酚红排空率并降低小肠酚红推进百分率。结论:云木香挥发油、水提液萃取物能够抑制豚鼠离体肠平滑肌的自主活动及拮抗乙酰胆碱所致痉挛状态下离体肠平滑肌;同时抑制正常小鼠胃排空,改善阿托品所致抑制状态下的小鼠胃排空障碍,拮抗新斯的明所致的小鼠胃排空和小肠推进功能亢进。
OBJECTIVE: To study the effects of volatile oil of Yunfu Oil and extracts of four kinds of water extracts on gastric emptying and small intestine prolapse in isolated guinea pig intestinal smooth muscle and mice. Methods: BL-420F bio-functional test system and modified phenol red content assay were used to observe the effects of volatile oil and extracts of four kinds of water extracts on gastric emptying and small intestine prolapse in isolated guinea pig intestinal smooth muscle and mice. Results: Volatile oil (4.0 × 10-2g crude drug / ml) and water extract (1.0 × 10-2g crude drug / ml) could significantly reduce the normal state of guinea pigs; volatile oil (2.0 × 10-2g crude drug / ml ), Aqueous extracts (1.5 × 10-2g crude drug / ml) can significantly reduce the average amplitude of guinea pig intestinal smooth muscle in spasm induced by Ach; volatile oil (2.4 × 10-2g crude drug / ml), water extract Extract (2.4 × 10-2g crude drug / ml) can significantly reduce the gastric mucosal red blood emptying rate of normal mice and significantly increase the gastric perfusion rate of gastric phenolphthalein in atropine-induced mice and significantly reduce the rate of neostigmine Due to hyperthyroidism mice gastric perfusion rate and reduce the rate of small intestine phenol red propulsion. Conclusion: The essential oil of Rhizoma et Radix notoginseng and its extract can inhibit the autonomic activity of isolated smooth muscle of guinea pig in vitro and antagonize the intestinal smooth muscle in vitro induced by acetylcholine. At the same time, it can inhibit the gastric emptying of normal mice and improve the inhibitory effect of atropine Under gastric emptying disorder in mice, the antagonism of neostigmine induced gastric emptying and intestinal propulsive function hyperthyroidism.