哺乳动物精子顶体中有一种蛋白分解酶,称顶体蛋白(acrosin)。目前常用手工涂明胶基质膜或放射自显影膜方法,根据单个精子周围的膜被消化所出现的晕,来检测顶体蛋白的活性。手工涂膜既费时又不易涂匀,由于膜不平整,晕可大小不一,甚至出现大面积无晕。放射自显影膜也存在膜厚不易被消化及银粒粗等缺陷。本文介绍一种快速和简便的机器涂膜法,制备的膜薄而均匀,易被快速消化,出现的晕大而清晰。该技术可用于诊断因顶体蛋白 Mammalian sperm acrosin has a protease, called acrosin. Currently commonly used manual gelatin stroma membrane or autoradiography method, according to a single sperm around the membrane is digested halo appear to detect acrosomal activity. Hand-painted film is not only time-consuming and difficult to paint evenly, due to uneven film, halo may vary in size, and even a large area without halo. Autoradiography film also exists in the film is not easy to be digested and silver particles and other defects. This article describes a fast and easy machine coating method, the preparation of thin and uniform film, easily digested quickly, appeared halo large and clear. This technique can be used to diagnose acrosomal proteins