Objective: To explore application of targeted contrast enhanced ultrasonography in diagnosis of early stage vascular endothelial injury and diabetic nephropathy. Methods: Targeted Sono VueTM microbubble was prepared by attaching anti-TM monoclonal antibody to the surface of ordinary micro-bubble Sono Vue by biotin-avidin bridge method and ultrasonic instrument was used to evaluate the developing situation of targeted microbubble in vitro. Twenty 12-week-old male GK rats and 20 Wistar rats were enrolled in this study, and were randomly divided into targeted angiography group and ordinary angiography group. Targeted microbubbles Sono VueTM or general microbubble Sono Vue were rapidly injected to the rats via tail vein; the developing situation of the two contrast agents in rats kidneys was dynamically observed. Time intensity curve was used to analyse rat kidney perfusion characteristics in different groups. Results: Targeted ultrasound microbubble Sono Vue-TM was successfully constructed, and it could be used to develop an external image. Targeted microbubbles Sono Vue-TM enabled clear development of experimental rat kidney. Time intensity curve shapes of rat kidney of the two groups showed as single apex with steep ascending and slowly descending branch. Compared with the control group, the rising slope of the GK rat renal cortex, medulla in targeted angiography group increased(P<0.05); the peak intensity of medulla increased(P<0.05), and the total area under the curve of medulla increased(P<0.05). Compared with control group, the ascending branch of the GK rat in renal cortex, medulla in ordinary angiography group increased(P<0.05). The peak intensity of the curve increased(P<0.05), and the total area under the curve increased(P<0.05). Compared with the ordinary angiography group, the peak of GK rat medullacurve in targeted angiography group intensity increased(P<0.05), and the total area under the curve increased(P<0.05). Conclusions: Targeted microbubbles Sono VueTM can make a clear development of experimental rat kidney, its stable performance meet the requirement of ultrasonic observation time limit, and it can reflect early changes of blood perfusion in GK rat kindey. Objective: To explore application of targeted contrast enhanced ultrasonography in diagnosis of early stage vascular endothelial injury and diabetic nephropathy. Methods: Targeted Sono Vue ™ microbubble was prepared by attaching anti-TM monoclonal antibody to the surface of ordinary micro-bubble Sono Vue by biotin- avidin bridge method and ultrasonic instrument was used to evaluate the developing situation of targeted microbubble in vitro. Twenty 12-week-old male GK rats and 20 Wistar rats were enrolled in this study, and were randomly divided into targeted angiography group and ordinary angiography group Targeted microbubbles Sono Vue ™ or general microbubble Sono Vue ™ were rapidly injected to the rats via tail vein; the developing situation of the two contrast agents in rats kidneys was significantly observed. Time intensity curve was used to analyze rat kidney perfusion characteristics in different groups. Results: Targeted ultrasound microbubble Sono Vue-TM was successfully constructe Targeted microbubbles Sono Vue-TM enabled clear development of experimental rat kidney. Time intensity curve shapes of rat kidney of the two groups showed as single apex with steep ascending and slowly descending branch. Compared with the control group, the rising slope of the GK rat renal cortex, medulla in targeted angiography group increased (P <0.05); the peak intensity of medulla increased (P <0.05), and the total area under the curve of medulla increased (P <0.05). Compared with control group, the ascending branch of the GK rat in renal cortex, medulla in ordinary angiography group increased (P <0.05). The peak intensity of the curve increased Compared with the ordinary angiography group, the peak of GK rat medullacurve in targeted angiography group intensity increased (P <0.05), and the total area under the curve increased (P <0.05). Conclusions: Targeted microbubbles Sono VueTM can make a clear development of experimental rat kidney, its stable performance meets the requirement of ultrasonic observation time limit, and it can reflect early changes of blood perfusion in GK rat kindey.