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建立了血、尿样品中巴比妥类药物的高效毛细管电泳分离测定方法，测定条件为：运行缓冲液配比：１００ｍｍｏｌ／Ｌ十二烷基硫酸钠－１００ｍｍｏｌ／Ｌ磷酸二氢钠－甲醇－水＝７０∶１５∶５∶１０（ｐＨ值调至７．５５±０．０５），操作电压１７ｋＶ，检测波长２００和２８５ｎｍ，２０ｍｉｎ内７种巴比妥类药物全部得到分离。考察并选择了体液样品的预处理方法，测定了血浆和尿液中６种巴比妥类药物浓度（苯巴比妥、甲基苯巴比妥、异戊巴比妥、硫喷妥、戊巴比妥和速可眠）。测定血药浓度的线性范围为５．０～３５μｇ／ｍｌ，尿样药物浓度线性范围为１．０～８．０μｇ／ｍｌ，最低检测浓度为１．０μｇ／ｍｌ，方法重现性为ＲＳＤ小于１３％。
A high performance capillary electrophoresis separation and determination method for barbiturates in blood and urine samples was established. The measurement conditions were: running buffer ratio: 100 mmol / L sodium dodecyl sulfate-100 mmol / L sodium dihydrogen phosphate-methanol- Water = 70:15:5:10 (pH adjusted to 7.55 ± 0.05), the operating voltage of 17kV, detection wavelength of 200 and 285nm, 7 kinds of barbiturates within 20min all were separated. The pretreatment methods for body fluid samples were investigated and selected. The concentrations of 6 barbiturates in plasma and urine (phenobarbital, methylphenobarbital, amobarbital, thiopental, Barbiturate and sleepless). The linear range of determination of plasma concentration was 5.0 ~ 35μg / ml, the linear range of urine drug concentration was 1.0 ~ 8.0μg / ml, the lowest detection concentration was 1.0μg / ml, the method reproducibility was RSD less than 13%.