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目的 通过分子改进提高βhCG CTP避孕疫苗的免疫原性。方法 根据分子设计及免疫识别原理 ,利用蛋白质固相合成技术人工合成一小分子表位嵌合多肽 ,内含 βhCG CTP上的两个B细胞表位 (β8、β9)以及一个源自麻疹病毒融合蛋白的T细胞表位 ,以此多肽免疫家兔 ,以WHO的βhCG CTP疫苗标准品为对照 ,用ELISA测定所产生的抗体滴度 ,比较两者的免疫原性。结果 该嵌合多肽免疫家兔后产生的抗体滴度显著高于WHOβhCG CTP疫苗标准品 ,且能与天然hCG结合。结论人工合成的表位嵌合多肽免疫原性较 βhCG CTP明显提高 ,而多肽分子的抗原性未受影响 ,免疫家兔后诱生的抗体与天然hCG结合力强 ,引入T细胞表位能提高小分子多肽的免疫原性 ,表明通过分子改进可提高避孕疫苗的免疫原性。
Objective To improve the immunogenicity of βhCG CTP contraceptive vaccine through molecular improvement. Methods According to the principle of molecular design and immune recognition, a small molecule epitope chimeric peptide was synthesized by protein solid-phase synthesis. It contained two B-cell epitopes (β8, β9) on βhCG CTP and one fusion protein derived from measles virus Protein T cell epitopes, the rabbit was immunized with this polypeptide. The WHO βhCG CTP vaccine standard was used as a control, and the antibody titer was measured by ELISA, and the immunogenicity of both was compared. Results The chimeric peptide immunized rabbits produced significantly higher antibody titers than the WHOβhCG CTP vaccine standard and were able to bind to native hCG. Conclusion The immunogenicity of the synthesized chimeric peptides was significantly higher than that of βhCG CTP, but the antigenicity of the peptides was not affected. The antibodies induced by immunized rabbits had strong binding ability with native hCG, and the introduction of T cell epitopes could be enhanced The immunogenicity of the small molecule polypeptide indicates that the immunogenicity of the contraceptive vaccine can be increased by molecular improvement.