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目的:观察复肾颗粒在肾间质纤维化过程中对转化生长因子β激活激酶(TAK1)表达的影响及其可能的肾脏保护作用机制。方法:采用单侧输尿管结扎(UUO)致肾间质纤维化大鼠模型,将60只大鼠随机分为6组:假手术组(N)、模型组(M,UUO组)、福辛普利组[F0,10 mg.kg-1.d-1]、复肾颗粒1组(F1,7.5 g.kg-1.d-1)、复肾颗粒2组(F2,15 g.kg-1.d-1)、复肾颗粒3组(F3,30 g.kg-1.d-1)。14 d后处死大鼠,取结扎侧肾组织采用HE及Masson染色观察病理变化,RT-PCR和Western-bloting检测肾组织TAK1和α-SMA的表达。结果:模型组大鼠肾纤维化程度及肾组织TAK1和α平滑肌肌动蛋白(α-SMA)的表达较假手术组显著升高(P<0.01),复肾颗粒各组及福辛普利组较模型组显著降低,尤以复肾颗粒2组为甚(P<0.01,P<0.05)。结论:复肾颗粒可能通过降低TAK1和α-SMA的含量而起到减轻肾间质纤维化病程进展的作用,推测其抑制TAK1和α-SMA表达上调的作用可能是其抗肾小管间质纤维化的机制之一。
Objective: To observe the effect of Fu Shen Granule on the expression of transforming growth factor-β activated kinase (TAK1) during renal interstitial fibrosis and its possible mechanisms of renal protection. Methods: Sixty male SD rats were randomly divided into 6 groups: sham operation group (N), model group (M, UUO group), Fosin P (F0, 10 mg.kg-1.d-1), Fufang Granule 1 (F1, 7.5 g.kg-1.d-1), Fufang Granule 2 1.d-1), Fu Shen particles three groups (F3, 30 g.kg-1.d-1). After 14 days, the rats were killed and the pathological changes were observed by HE staining and Masson staining. The expressions of TAK1 and α-SMA in renal tissues were detected by RT-PCR and Western-bloting. Results: The degree of renal fibrosis and the expression of TAK1 and α-SMA in renal tissue in model group were significantly higher than those in sham-operation group (P <0.01). Fufang granules and fosinopril Group than the model group was significantly lower, especially Fu Shen particles in two groups (P <0.01, P <0.05). Conclusion: Fu Shen Granule may play a role in alleviating the progression of renal interstitial fibrosis by decreasing the content of TAK1 and α-SMA. The suppressive effect of Fu Shen Granule on the up-regulation of TAK1 and α-SMA may be due to its anti-tubulointerstitial fibrosis One of the mechanisms.