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本研究采用大鼠评价雷公藤配伍甘草降低肝毒性的效果,并应用代谢组学技术手段分析这一减毒过程体内小分子代谢物的变化及可能涉及到的代谢通路。前期通过不同分组大鼠给予雷公藤或雷公藤配伍甘草的实验干预,观察大鼠的一般状态、肝组织病理改变、肝功能生化指标及炎症因子的变化。结果显示,雷公藤组肝组织损伤明显,配伍后肝组织未见明显损伤;生化指标及炎症因子也提示雷公藤配伍甘草后可有效降低其肝毒性,由此可见,雷公藤配伍甘草对肝脏起到一定的减毒保护作用;随后采用HPLC-MS/MS-IT-TOF对各组大鼠血清代谢差异进行表征,通过多元统计分析方法筛选出脂肪酸、甘油酯、甘油磷酸、磷脂酰乙醇胺和磷脂酰胆碱等15个潜在生物标志物,主要涉及甘油磷脂代谢、亚油酸代谢、α-亚油酸代谢和糖基磷脂酰肌醇末端生物合成等7个代谢通路。基于上述实验结果,本文发现雷公藤配伍甘草能有效降低其肝毒性,甘油磷脂代谢可能是雷公藤肝毒性及配伍甘草减毒的关键代谢通路,从而为实现减毒调控提供参考。
In this study, we evaluated the effect of Tripterygium wilfordii and Glycyrrhiza uralensis on reducing hepatotoxicity. Metabolomic techniques were used to analyze the changes of small molecule metabolites and possible metabolic pathways involved in this attenuation process. In the early stage, different groups of rats were given tripterygium wilfordii or Tripterygium compatibility licorice experimental intervention to observe the general state of rats, pathological changes of liver tissue, liver biochemical indicators and changes in inflammatory cytokines. The results showed that the Tripterygium wilfordii group of liver injury significantly, no significant liver damage after compatibility; biochemical indicators and inflammatory cytokines also showed that compatibility with licorice can reduce its liver toxicity, we can see that Tripterygium compatibility of licorice on the liver To a certain degree of attenuated protection; then using the HPLC-MS / MS-IT-TOF to characterize the difference of serum metabolism in each group of rats, and to screen the fatty acids, glycerides, glycerophosphate, phosphatidylethanolamine and phospholipid by multivariate statistical analysis 15 potential biomarkers such as acetylcholine, which mainly involved seven metabolic pathways, such as glycerophospholipid metabolism, linoleic acid metabolism, α-linoleic acid metabolism and glycosylphosphatidylinositol terminal biosynthesis. Based on the above experimental results, this article found that Tripterygium compatibility licorice can effectively reduce the liver toxicity, glycerophospholipids metabolism may Tripterygium Hepatotoxicity and compatible licorice attenuated key metabolic pathways, and thus provide a reference for the attenuation of regulation.