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目的探讨人类活体胎儿血循环中的造血干/祖细胞(HSPC)表面标记物CD133、CD34、CD38随孕龄变化的规律。方法通过B超引导下脐静脉穿刺术等方法获得妊娠12~41周活体胎儿血106例,应用双色免疫荧光标记法分别标记单个核细胞CD34、CD133抗原和CD34、CD38抗原,流式细胞仪检测抗原表达。结果所有在B超引导下胎儿标本取样术后母胎未出现严重并发症,CD34+细胞/有核细胞、CD38-细胞/CD34+细胞、CD133+细胞/有核细胞、CD133+细胞/CD34+细胞含量均随孕周增加而降低,且与孕周呈直线负相关关系,其变化范围分别为4·21%~0·04%、58·5%~10·7%、3·69%~0·31%、87·6%~48·5%。结论越早期的胎儿循环血HSPC在免疫表型上越原始,这可能是宫内基因治疗理想的靶细胞。
Objective To investigate the regularity of the changes of surface markers CD133, CD34 and CD38 of hematopoietic stem / progenitor cells (HSPCs) in human fetal blood circulation with gestational age. Methods B-ultrasonography-guided umbilical vein puncture and other methods to obtain the pregnancy of 12 to 41 weeks of fetal blood in 106 cases, the use of two-color immunofluorescence labeling of mononuclear cells CD34, CD133 antigen and CD34, CD38 antigen, flow cytometry Antigen expression. Results All the fetuses did not have serious complications after fetus sample fetching B ultrasound. The content of CD34 + cells, CD38- / CD34 + cells, CD133 + cells / nucleated cells and CD133 + cells / CD34 + And decreased linearly with gestational age. The range of change was 4.21% -0.04%, 58.5% -10.7%, 3.69% -0.31%, 87 · 6% ~ 48 · 5%. Conclusion The earlier fetal circulating blood HSPC is more primitive in the immunophenotype, which may be the ideal target cell for intrauterine gene therapy.