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目的 探讨熊去氧胆酸(UDCA)对雌激素诱发孕鼠肝内胆汁淤积的作用。方法 孕15d大鼠30只随机分成3组,对照组用丙二醇(PG),EE组用乙炔雌二醇(EE),UDCA组用UDCA+EE。用药前、用药后3和5d测谷丙转氨酶(ALT)及谷草转氨酶(AST)、碱性磷酸酶(ALP)、胆固醇(CHO)、甘油三酯(TG)、总胆酸(TBA),同时观察肝脏形态学变化及胎鼠生长发育情况。结果 用药后EE组的孕鼠ALT、ALP、AST及TBA明显升高(P<0.05),CHO和TG明显下降(P<0.05),对照组及UDCA组上述生化指标用药前后无明显变化(P>0.05)。EE组孕鼠肝脏在光镜和电镜下可见毛细胆管内胆汁酸沉积,对照组及UDCA组肝脏无类似改变。EE组胎仔的体重、身长、尾长较对照组有明显差异(P<0.05);EE组大鼠孕期为19d,对照组及UDCA组大鼠孕期为22和21d,两者有明显差异(P<0.05)。结论 UDCA可预防用EE处理的孕鼠发生肝内胆汁淤积样改变
Objective To investigate the effect of ursodeoxycholic acid (UDCA) on intrahepatic cholestasis induced by estrogen in pregnant rats. Methods Thirty pregnant rats were randomly divided into three groups at the age of 15 days. Propylene glycol (PG) was used in the control group, ethinyl estradiol (EE) in the EE group and UDCA + EE in the UDCA group. Before treatment, ALT, AST, ALP, CHO, TG and TBA were measured at 3 and 5 days after treatment. Morphological changes of liver and fetal growth and development were observed. Results The levels of ALT, ALP, AST and TBA were significantly increased (P <0.05) and the levels of CHO and TG were significantly decreased in EE group (P <0.05), while those in control group and UDCA group did not change significantly > 0.05). In EE group, the liver of pregnant rats showed capillary bile acid deposition under light microscope and electron microscope. There was no similar change in liver of control group and UDCA group. The body weight, body length and tail length of EE group were significantly different from those of control group (P <0.05). The pregnancy rate of EE group was 19 days and that of control group and UDCA group was 22 and 21 days <0.05). Conclusion UDCA prevents intrahepatic cholestasis-like changes in pregnant rats treated with EE