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目的考察非离子表面活性剂维生素E聚乙二醇1000琥珀酸酯(TPGS)对宝藿苷I抑制乳腺癌细胞MCF-7增殖的影响。方法 MTT法测定宝藿苷I对MCF-7的细胞毒性,用荧光显微镜观察宝藿苷I的细胞摄取,并采用HPLC法测定细胞内的宝藿苷I的量。结果在应用TPGS后,宝藿苷I对MCF-7增殖的抑制作用增强,在宝藿苷I低浓度的情况下作用更显著;当宝藿苷I与TPGS质量比分别为1∶1、1∶2、1∶4时,在MCF-7细胞培养2 h的摄取率分别为29.51%、38.12%、40.37%,与仅用宝藿苷I相比分别提高了27.92%、65.24%、74.99%。结论 TPGS能够增加MCF-7对宝藿苷I的细胞摄取,并增强其对MCF-7的细胞毒作用。
Objective To investigate the effect of nonionic surfactant vitamin E polyethylene glycol 1000 succinate (TPGS) on the inhibition of the proliferation of breast cancer cell MCF-7 by Poisonin I. Methods MTT method was used to determine the cytotoxicity of treasure glycoside I to MCF-7 cells. The cellular uptake of bioactive glycosides I was observed with fluorescence microscope. The content of bioactive glycosides I was determined by HPLC. Results After application of TPGS, the inhibitory effect of treasure glycoside I on the proliferation of MCF-7 was enhanced, and the effect was more significant at the low concentration of treasure glycoside I. When the ratio of treasure glycinin I to TPGS was 1: 1, 1 2, 1: 4, the uptake rates of MCF-7 cells cultured for 2 h were 29.51%, 38.12% and 40.37%, respectively, increased by 27.92%, 65.24% and 74.99% . Conclusion TPGS can increase the cellular uptake of icariin I by MCF-7 and enhance its cytotoxicity to MCF-7.