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业已证明,处于晚G_1期或S期的细胞经43℃/1小时处理后,与未经高温处理的细胞相比,起始作用的DNA片段减少,DNA延伸过程也受到抑制。本文旨在进一步研究高温和电离辐射对于DNA复制起始作用和延伸过程的影响。本实验评价DNA复制(起始作用和延伸作用)改变所采用的为pH逐级碱洗脱法和碱性蔗糖梯度技术。实验材料为同步化单层培养的中国地鼠卵巢细胞。高温处理为水浴43℃,1小时。X射线的剂量为5Gy,由4MeV线性加速器产生,以剂量率4Gy/分照射细胞。
It has been demonstrated that after treatment at 43° C./1 hour in cells in the late G 1 or S phase, the initial DNA fragments are reduced and DNA extension is inhibited compared to non-hyperthermia treated cells. This article aims to further investigate the effects of high temperature and ionizing radiation on DNA replication initiation and extension processes. This experiment evaluated changes in DNA replication (initiation and elongation) using pH-gradient alkaline elution and basic sucrose gradient techniques. The experimental material was synchronized monolayer culture of Chinese hamster ovary cells. The high temperature treatment was a water bath at 43°C for 1 hour. The dose of X-rays was 5 Gy, produced by a 4 MeV linear accelerator, and irradiated at a dose rate of 4 Gy/min.