目的： 建立人血浆中己酮可可碱浓度测定法。方法：采用反相高效液相色谱法。结果： 方法准确度为８２６ ％ ～１０５９ ％ （２５ ～３００ ｎｇ／ ｍｌ） ；批内 Ｒ Ｓ Ｄ 分别为７６ ％ （５０ ｎｇ／ｍｌ） 、５８ ％ （１００ ｎｇ／ｍｌ） 和３９ ％ （１５０ ｎｇ／ｍｌ） ，批间 Ｒ Ｓ Ｄ 分别为１０８ ％ （５０ ｎｇ／ ｍｌ） 、７９ ％ （１００ ｎｇ／ ｍｌ） 和４１ ％ （１５０ ｎｇ／ ｍｌ） ；最低检测浓度为２５ ｎｇ／ ｍｌ（ Ｓ／ Ｎ＝ ３） 。结论：本法准确、可靠、灵敏，适用于己酮可可碱新剂型的生物利用度和药代动力学研究。 Objective: To establish a method for the determination of pentoxifylline in human plasma. Methods: Reversed-phase high performance liquid chromatography. Results The accuracy of the method was 826% -1059% (25 ~ 300 ng / ml). The intra-operative R SD was 76% (50 ng / ml) and 58% (100 ng / ml) and 39% (150 ng / ml) respectively. The inter-assay RSD was 108% (50 ng / ml), 79% (100 ng / ml) and 41% / Ml); the lowest detection concentration was 25 ng / ml (S / N = 3). Conclusion: This method is accurate, reliable, sensitive and suitable for the study of bioavailability and pharmacokinetics of pentoxifylline.