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本文对11例诊断困难或有争议的胃恶性肿瘤进行了免疫组织化学标记及分析,均获明确诊断。现报道如下。材料和方法11例为近年来本院及沈阳市第八医院病理科的胃肿瘤标本。均为弥漫成片的小圆细胞或多角形细胞肿瘤。初诊恶性淋巴瘤3例,未分化瘤2例,两者难以鉴别者5例,浆细胞肉瘤1例。所有组织用常规10%福尔马林固定、石蜡包埋的方法处理。各例均做 HE 染色及三种免疫组织化学标记。试剂:白细胞共同抗原(LCA)、癌胚抗原(CEA)、抗角蛋白抗体(Keratin),丹麦 DAK0公司提供羊抗兔 IgG、兔 PAP(北京生物制品所提供)、羊抗鼠 IgG、鼠 PAP(无锡制药二厂提供)。
In this article, 11 cases of difficult or controversial gastric malignancies were immunohistochemically labeled and analyzed. The report is as follows. Materials and Methods 11 cases were gastric cancer specimens of the Department of Pathology in this hospital and the No.8 Hospital of Shenyang in recent years. All are diffuse small round cells or polygonal cell tumors. There were 3 cases of newly diagnosed malignant lymphomas, 2 cases of undifferentiated tumors, 5 cases of difficult to differentiate, and 1 case of plasma cell sarcoma. All tissues were treated with a conventional 10% formalin-fixed, paraffin-embedded method. Each case was stained with HE and three immunohistochemical markers. Reagents: leukocyte common antigen (LCA), carcinoembryonic antigen (CEA), anti-keratin antibody (Keratin), Danish anti-rabbit IgG provided by Denmark DAK0, rabbit PAP (provided by Beijing Biological Products), goat anti-mouse IgG, rat PAP (Wuxi No. 2 Pharmaceutical Factory).