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【目的】为明确韭菜迟眼蕈蚊Bradysia odoriphaga对新烟碱类杀虫剂的抗性水平及其抗性机制。【方法】通过测定不同地区韭菜迟眼蕈蚊对3种新烟碱类杀虫剂吡虫啉、噻虫嗪和噻虫胺的敏感度,及通过增效剂实验和酶活性测定,初步探索抗性产生机制,为韭菜迟眼蕈蚊抗性治理提供依据。【结果与结论】4个不同的韭菜迟眼蕈蚊田间种群对3种新烟碱类杀虫剂均产生了不同水平的抗性。其中,唐山种群对3种新烟碱杀虫剂均产生了较高的抗性。研究发现,唐山种群的7-乙氧基香豆素-O-脱乙基酶(ECOD)比活力为(3.89±0.31)pmol/(mg·pro·min),显著高于敏感品系。增效剂PBO对唐山种群的吡虫啉毒力的增效比为2.64,高于对敏感品系的增效比1.08。因此,P450s酶活性的升高与韭菜迟眼蕈蚊对吡虫啉的抗性有关。
【Objective】 The purpose of this study was to determine the resistance of Neisseria gonorrhoeae Bradysia odoriphaga to neonicotinoid insecticides and its mechanism of resistance. 【Method】 The sensitivity of three new neonicotinoid insecticides, imidacloprid, thiamethoxam and clothianidin, to fungus Myrmecidae in different areas was determined and their synergistic effects on the resistance Produce mechanism for the leek Davis mosquito resistance control provide the basis. [Results and Conclusions] Four different Leek larvae were found to have different levels of resistance to the three neonicotinoid insecticides. Among them, Tangshan population had higher resistance to three neonicotinoid insecticides. The results showed that the specific activity of 7-ethoxycoumarin-O-deethylase (ECOD) in Tangshan population was (3.89 ± 0.31) pmol / (mg · pro · min), which was significantly higher than that of the susceptible strain. The synergistic ratio of synergist PBO to imidacloprid in Tangshan population was 2.64, which was higher than that of the susceptible strain (1.08). Therefore, the increase of P450s enzyme activity is related to the resistance of imidacloprid to Lepidoptera frugiperda.