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目的探讨哮喘大鼠肾上腺皮质功能变化与白细胞介素10(IL-10)水平的关系。方法卵蛋白致敏方法制备大鼠哮喘模型,随机分为哮喘急性发作期组(A组)、哮喘缓解期组(B组)、正常对照组(C组)、模型对照组(D组),每组6只。放射免疫分析法测定血浆皮质酮(CORT)、促肾上腺皮质激素(ACTH)以及下丘脑促肾上腺皮质激素释放激素(CRH)的含量;ELASA法检测下丘脑组织和血浆IL-10的水平。结果与C组比较,A组大鼠脑组织和血浆IL-10水平均下降[(15.45±4.06)pg.mg-1.prot-1vs.(28.74±8.91)pg.mg-1.prot-1,和(26.62±6.78)pg/mlvs.(54.86±14.32)pg/ml](P<0.01);CORT、ACTH以及CRH的含量降低[(1.47±0.11)ng.mg-1.prot-1vs.(1.84±0.12)pg.mg-1.prot-1,(75.55±8.17)pg/mlvs.(108.34±16.57)pg/ml,和(121.69±17.65)ng/mlvs.(162.01±21.21)ng/ml](P<0.01)。哮喘缓解期大鼠脑组织IL-10水平上升,CRH的含量增高。结论哮喘大鼠存在肾上腺皮质功能减退可能与下丘脑和血中IL-10水平变化有关。
Objective To investigate the relationship between changes of adrenal cortex function and interleukin 10 (IL-10) levels in asthmatic rats. Methods Asthmatic rat models were established by sensitization with ovalbumin. The asthma models were randomly divided into three groups: acute asthma exacerbation group (A group), asthma remission group (B group), normal control group (C group), model control group (D group) 6 per group Radioimmunoassay was used to determine the contents of corticosterone (CORT), adrenocorticotropic hormone (ACTH) and hypothalamic Corticotropin Releasing Hormone (CRH). ELASA was used to detect the levels of IL-10 in hypothalamus and plasma. Results Compared with group C, the levels of IL-10 in brain tissue and plasma in group A were decreased [(15.45 ± 4.06) pg.mg-1.prot-1vs. (28.74 ± 8.91) pg.mg-1.prot-1 , And (26.62 ± 6.78) pg / ml vs. (54.86 ± 14.32) pg / ml] (P <0.01). The levels of CORT, ACTH and CRH were decreased in [(1.47 ± 0.11) ng.mg-1.prot-1vs. (1.84 ± 0.12) pg.mg-1.prot-1, (75.55 ± 8.17) pg / ml vs 108.34 ± 16.57 pg / ml and (121.69 ± 17.65) ng / ml vs ml] (P <0.01). The level of IL-10 in brain tissue of asthmatic remission rats increased and the content of CRH increased. Conclusion The existence of adrenal insufficiency in asthmatic rats may be related to the changes of IL-10 in hypothalamus and blood.