目的　建立人骨骼肌干细胞体外分裂分化的模式。方法　人工流产胎儿肌肉组织用胰酶和胶原酶联合消化后 ,经percoll密度梯度离心分离骨骼肌干细胞 (又称卫星细胞 )。并以Matrigel为基质 ,在体外培养条件下观察其分裂与分化。结果　经percoll密度梯度离心 ,能将干细胞与成纤维细胞分离 ,纯度达 80 %以上。干细胞具有分裂、分化的潜能 ,在体外由成肌细胞增殖 ,经彼此融合成为肌管细胞 ,最终形成多核且具有收缩能力的骨骼肌细胞。上述分裂、分化过程历时 2 1天 ,较啮齿类长。结论　人胎儿骨骼肌干细胞在体外具有分裂、分化潜能。因此 ,干细胞可以用作细胞移植和基因治疗的靶组织 Objective To establish a model of human skeletal stem cell differentiation in vitro. Methods Aborted fetus muscle tissue was digested with trypsin and collagenase, and then skeletal muscle stem cells (also called satellite cells) were separated by percoll density gradient centrifugation. Matrigel was used as matrix to observe its division and differentiation under in vitro culture conditions. Results The percoll density gradient centrifugation, stem cells and fibroblasts can be separated with a purity of more than 80%. Stem cells have the potential to divide and differentiate, proliferate in vitro by myoblasts, fuse with each other to become myotube cells, and eventually form multi-nuclear and contractile skeletal muscle cells. The division and differentiation process lasted 21 days, longer than rodents. Conclusion Human fetal skeletal muscle stem cells have the potential of dividing and differentiating in vitro. Therefore, stem cells can be used as target tissues for cell transplantation and gene therapy