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目的:探讨附子水溶性生物碱对急性心力衰竭(AHF)大鼠的治疗作用。方法:采用盐酸普罗帕酮复制大鼠急性心力衰竭模型,采用BL-420系统记录大鼠心率、心室内压,随后静脉注射附子水溶性生物碱1.25×10-3、2.50×10-3、5.00×10-3g/kg(注射液中提取物含量0.05g/ml),或阳性药物参麦注射液6.67g/kg,记录给药5、10、20、30、60min时的HR、+dp/dtmax及-dp/dtmax的变化;另取一批动物,采用相同方法给药,并于给药后20min取血,采用酶联免疫法(ELISA)测定血清Ang-Ⅰ、Ang-Ⅱ、ANP、BNP、ALD含量。结果:附子水溶性生物碱1.25×10-3、2.50×10-3、5.00×10-3g/kg静脉给药,可有效升高模型大鼠的心率和LV+dp/dtmax,降低LV-dp/dtmax;且能降低AHF大鼠血清Ang-Ⅱ、TNF-α、ANP、BNP、ALD水平。结论:附子水溶性生物碱具有强心作用,治疗急性心力衰竭的机制与调节肾素-血管紧张素-醛固酮系统(RAAS)有关。
Objective: To investigate the therapeutic effect of aconite water-soluble alkaloids on acute heart failure (AHF) rats. Methods: Rat model of acute heart failure was induced by propafenone hydrochloride. Heart rate and ventricular pressure were recorded by BL-420 system. Then aqueous solution of aconitine alkaloids 1.25 × 10-3, 2.50 × 10-3, 5.00 × 10-3g / kg (injection content of 0.05g / ml), or the positive drug Shenmai injection 6.67g / kg, recorded at 5,10,20,30,60 min HR, dp / dtmax and -dp / dtmax. Another group of animals were given the same method and the blood samples were taken 20min after administration. The serum levels of Ang-Ⅰ, Ang-Ⅱ, ANP were measured by enzyme-linked immunosorbent assay (ELISA) BNP, ALD content. Results: The aconite water-soluble alkaloids 1.25 × 10-3,2.50 × 10-3,5.00 × 10-3g / kg intravenously, can effectively increase the heart rate and LV + dp / dtmax of model rats, reduce the LV-dp / dtmax; and can reduce the level of serum Ang-Ⅱ, TNF-α, ANP, BNP and ALD in AHF rats. CONCLUSION: The aconite water-soluble alkaloids have cardiotonic effect and the mechanism of treatment of acute heart failure is related to the regulation of renin-angiotensin-aldosterone system (RAAS).