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目的:研究黄连解毒汤对大鼠胶质瘤C6细胞增殖、凋亡、迁移和侵袭的影响及作用机制。方法:分别采用MTT法、流式细胞术、划痕法和Transwell法测定黄连解毒汤对大鼠胶质瘤C6细胞增殖、凋亡、迁移和侵袭的影响,分别采用RT-PCR和Western blot法测定黄连解毒汤对大鼠胶质瘤C6细胞中BCL-2、BAX、Caspase-3 mRNA及蛋白表达的影响。结果:黄连解毒汤100、300、900 mg/L作用大鼠C6胶质瘤细胞24 h或48 h时可显著抑制细胞增殖(P<0.01),并呈浓度依赖性(P<0.01);黄连解毒汤100、300、900 mg/L作用大鼠C6细胞24 h时的凋亡率均较空白对照组显著升高(P<0.01),划痕刻度均较空白对照组显著变宽(P<0.01),穿膜细胞数量均较空白对照组显著减少(P<0.01),BCL-2 mRNA及蛋白表达显著降低,而BAX和Caspase-3 mRNA及蛋白表达显著升高(P<0.01),并呈浓度依赖性(P<0.01)。结论:黄连解毒汤可抑制大鼠胶质瘤C6细胞的增殖、迁移和侵袭,促进C6细胞凋亡,其机制可能与抑制C6细胞BCL-2表达以及促进BAX和Caspase-3表达有关。
Objective: To investigate the effects of Huanglian Jiedu Decoction on the proliferation, apoptosis, migration and invasion of C6 glioma cells and its mechanism. Methods: The effects of Huanglian Jiedu Decoction on the proliferation, apoptosis, migration and invasion of C6 glioma cells were determined by MTT method, flow cytometry, scratch assay and Transwell method. RT-PCR and Western blot To determine the effect of Huanglian Jiedu Decoction on the expressions of BCL-2, BAX, Caspase-3 mRNA and protein in C6 glioma cells. Results: Huanglian Jiedu decoction could inhibit the proliferation of C6 glioma cells at 100, 300, 900 mg / L for 24 h or 48 h (P <0.01) and in a concentration - dependent manner (P <0.01) The apoptosis rate of rat C6 cells treated with detoxification decoction of 100, 300 and 900 mg / L for 24 h was significantly higher than that of the blank control group (P <0.01), and the scratch scale was significantly wider than that of the blank control group (P < 0.01). The number of transmembrane cells was significantly decreased (P <0.01), the expression of BCL-2 mRNA and protein was significantly decreased, while the expressions of BAX and Caspase-3 mRNA and protein were significantly increased (P <0.01) In a concentration-dependent manner (P <0.01). Conclusion: Huanglian Jiedu Decoction can inhibit the proliferation, migration and invasion of C6 glioma cells and promote the apoptosis of C6 cells. Its mechanism may be related to inhibiting the expression of BCL-2 and promoting the expression of BAX and Caspase-3 in C6 glioma cells.