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目的探讨长期丘脑底核脑深部电刺激对猴帕金森病模型纹状体区细胞外液中γ-氨基丁酸含量的影响。方法建立偏侧猴帕金森病模型,在患侧丘脑底核植入人用脑深部刺激电极,在双侧壳核、尾状核头部植入微透析探针,同期猴背部皮下植入脉冲发生器,通过皮下导线与刺激电极相连。通过测试后给予有效高频电刺激,分别在打开脉冲发生器前和开机后的不同时间点应用持续微透析的方法对纹状体细胞外液进行取样(开机后1周、1、8、12个月)。应用高效液相色谱荧光法检测开机前后的尾状核和壳核细胞外液的γ-氨基丁酸的含量变化。结果在丘脑底核慢性高频电刺激下,电极侧尾状核及壳核的γ-氨基丁酸含量在开机后1周、1个月时均较开机前明显增加(P<0.05),但在开机后8、12个月时γ-氨基丁酸含量较开机前、开机1周、1个月时又明显下降(P<0.05)。结论丘脑底核脑深部刺激后可引起纹状体区细胞外液中γ-氨基丁酸的含量短期内明显升高,但长期的刺激后其含量会明显下降。这可能是丘脑底核脑深部电刺激的作用机制之一。
Objective To investigate the effects of long-term subthalamic nucleus deep brain stimulation on γ-aminobutyric acid (GABA) content in striatum of monkey model of Parkinson’s disease. Methods A hemipark monkey model of Parkinson’s disease was established. Human deep brain stimulation electrodes were implanted into the hypothalamus of the affected side. Microdialysis probes were implanted into the nucleus of both putamen and caudate nucleus. The back of the monkey was subcutaneously implanted with pulses Generator, connected to the stimulation electrode via a subcutaneous lead. After the test to give effective high-frequency electrical stimulation, respectively, before opening the pulse generator and the boot time at different time points by continuous microdialysis of striatal extracellular fluid sampling (1 week after turning on, 1,8,12 Month). The contents of γ-aminobutyric acid in caudate and putamen fluid before and after switching on were detected by high performance liquid chromatography (HPLC). Results After chronic high frequency electrical stimulation of the subthalamic nucleus, the content of γ-aminobutyric acid in caudate putamen and putamen increased significantly (P <0.05) at 1 week and 1 month after starting up, respectively At 8 and 12 months after start-up, the content of γ-aminobutyric acid decreased significantly (P <0.05) compared with that before power-on and power-on for 1 week and 1 month. Conclusions Deep brain stimulation of the subthalamic nucleus causes a significant increase in the content of γ-aminobutyric acid in the extracellular fluid of the striatum in the short term, but its content decreases significantly after long-term stimulation. This may be one of the mechanisms of deep brain stimulation in the subthalamic nucleus.