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目的:HPLC/外标法测定银杏叶中聚戊烯醇类化合物(PPAs,PPs)含量。方法:银杏叶样品10 g,加入石油醚100mL,室温下超声萃取45 min,共4次。提取物硅胶柱层析(硅胶100～140目,柱15 mm×350 mm),用石油醚-乙酸乙酯(90:10)洗脱。样品溶液采用HPLC分析:Inertsil ODS-3柱(4.6 mm×250 mm),柱温40℃,异丙醇-甲醇-正己烷-水(250:125:75:10)为流动相,流速1 mL·min~(-1),紫外检测器,检测波长215 nm。结果:聚戊烯醇类化合物中各组分基线分离,可同时分析银杏叶中PPAs、PPs含量,PPAs(或 PPs)进样量在0.26～5.2μg范围内与峰面积呈线性关系,PPAs、PPs平均加样回收率分别为98.8％和97.4％,RSD分别为1.8％和2.4％。结论:样品处理简单,结果稳定、准确,可作为银杏叶中聚戊烯醇类化合物的测定方法。
Objective: To determine the content of polyprenols (PPAs, PPs) in Ginkgo biloba leaves by HPLC/external standard method. Method: 10 g of Ginkgo biloba leaf sample was added with 100 mL of petroleum ether and extracted by ultrasound at room temperature for 45 min for 4 times. The extract was subjected to silica gel column chromatography (silica gel 100-140 mesh, column 15 mm x 350 mm) and eluted with petroleum ether-ethyl acetate (90:10). The sample solution was analyzed by HPLC: Inertsil ODS-3 column (4.6 mm × 250 mm), column temperature 40°C, isopropyl alcohol-methanol-n-hexane-water (250:125:75:10) as mobile phase, flow rate 1 mL · min~(-1), UV detector, detection wavelength 215 nm. RESULTS: Baseline separation of various components of polyprenols could simultaneously analyze the contents of PPAs and PPs in Ginkgo biloba leaves. The injection volume of PPAs (or PPs) was linear with the peak area in the range of 0.26-5.2 μg. PPAs, The average recovery of PPs was 98.8% and 97.4%, respectively, and RSD was 1.8% and 2.4%, respectively. Conclusion: The sample processing is simple and the result is stable and accurate. It can be used as a method for the determination of polyprenols in Ginkgo biloba leaves.