柠檬酸测定有气相色谱法、化学法、酶法等 ,多用于食品、药品测定。血清柠檬酸测定在临床上很少应用 ,在氟乙酰胺中毒中应用国内未见报道。根据氟乙酰胺中毒机制推断 ,柠檬酸在体内代谢发生障碍并积聚 ,但是否在血液中增加 ,目前尚有不同意见[1 4 ] 。为此 ,我们对它的酶法进行 Determination of citric acid gas chromatography, chemical methods, enzymes, etc., used for food, medicine determination. Serum citric acid assay is rarely used in clinical applications in fluoroacetamide poisoning has not been reported in China. According to the mechanism of fluoroacetamide inference that citric acid metabolism in the body obstacles and accumulation, but whether in the blood increased, there are different views [14]. To this end, we conducted its enzymatic method