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目的研究藤甲酰苷的急性毒性、遗传毒性和大鼠Ⅱ阶段的生殖毒性。方法急性毒性实验采用Bliss法,通过300、250、100、50、25 mg·kg-1五个剂量组测定昆明小鼠半数致死量;遗传毒性设置Ames实验和微核实验,其中Ames实验采用平板掺入法,设置500、100、20、4、0.8μg五个剂量组,小鼠微核试验设65、32.5、16.25 mg·kg-1三个剂量组;生殖毒性实验通过43、8.7、4.35 mg·kg-1三个剂量组考察大鼠致畸情况。结果藤甲酰苷对小鼠半数致死量为129.77 mg·kg-1,其95%可信限为93.289~179.38 mg·kg-1;Ames实验中受试物各剂量组回变菌落数均未超过生理盐水组2倍,且无剂量-反应关系;微核试验与生理盐水组之间无显著差异,结果均为阴性;致畸实验与生理盐水组相比,8.7和4.35 mg·kg-1剂量组对大鼠的胚胎形成、胎鼠的发育、骨骼及内脏无明显影响(P>0.05),43 mg·kg-1剂量组有显著差异(P<0.05)。结论藤甲酰苷属中等毒性化合物,在本实验剂量范围内藤甲酰苷不具有遗传毒性。8.7和4.35 mg·kg-1剂量组无致畸胎毒性,而43 mg·kg-1剂量组有一定的致畸毒性。
Aim To study the acute toxicity, genotoxicity and genotoxicity of rat venom Ⅱ in rats. Methods Acute toxicity test using the Bliss method, through the five doses of 300,250,100,50,25 mg · kg-1 determination of Kunming mice half-lethal dose; genotoxicity set Ames test and micronucleus test, which Ames test using plate Spiked with five doses of 500, 100, 20, 4 and 0.8μg, micronucleus test of mice with three doses of 65, 32.5 and 16.25 mg · kg-1 respectively. The reproductive toxicity test passed 43,8.7,4.35 mg · kg-1 three dose group to investigate the teratogenicity of rats. The results showed that Parthenoylglycoside had a half-lethal dose of 129.77 mg · kg-1 and a 95% confidence limit of 93.289-179.38 mg · kg-1. In the Ames experiment, There was no dose-response relationship between the micronucleus test group and the saline group, and the result was negative. The teratogenicity test was significantly higher than the normal saline group (8.7 and 4.35 mg · kg -1 The dose group had no significant effect on embryo formation, fetal rat development, skeletal and visceral (P> 0.05), 43 mg · kg-1 dose group (P <0.05). Conclusions Formic acid is a moderately toxic compound, which is not genotoxic in the experimental dose range. The dose of 8.7 and 4.35 mg · kg-1 had no teratogenicity, while the group of 43 mg · kg-1 had some teratogenicity.