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近年,对HBV-DNA的结构与功能的研究进入了新的水平,HBV-DNA的前s基因区(简称Pre-s区)调控的基因产物前s多肽(简称Pre-s多肽)与人体多聚白蛋白结合活性(简称PHSA-B)有密切关系。该问题1974年首先由Lenkei提出,以后学者对PHSA-B与乙型肝炎的关系做了深入研究。意大利学者最近就PHSA-B在甲型肝炎和非甲非乙型肝炎关系做了进一步研究。Pillot实验室报告用CHO细胞重组的含HBV的Pre-s_2基因克隆所得的Pre-s_2多肽为抗原,免疫小鼠得到抗Pre-s_2单克隆抗体,使用该抗原抗体系统建立了酶标检测方法,发现对急性肝炎预后判断十分敏感并与DNAP、HBV-DNA及e系统的
In recent years, the study on the structure and function of HBV-DNA has entered a new level. The pre-s gene (Pre-s polypeptide) regulated by the pre-s region of HBV-DNA (Pre-s region) Poly albumin binding activity (referred to as PHSA-B) are closely related. This question was first proposed by Lenkei in 1974 and later scholars conducted a thorough study of the relationship between PHSA-B and hepatitis B. Italian scholars recently conducted a further study on the relationship between PHSA-B in hepatitis A and non-A, non-B hepatitis. Pillot Labs reported that pre-s2 polypeptide obtained by cloning of Pre-s_2 gene of HBV recombined by CHO cells was used as antigen and the anti-Pre-s2 monoclonal antibody was obtained from the mice immunized with the anti-Pre-s_2 antibody. The ELISA method was used to establish the enzyme- Found to be very sensitive to the prognosis of acute hepatitis and DNAP, HBV-DNA and e system