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目的:研究口腔鳞癌组织血管内皮生长因子(VEGF)、诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)的表达与癌细胞增殖的相关性。方法：应用免疫组化方法检测64例口腔鳞癌手术切除标本VEGF、iNOS、eNOS和增殖细胞核抗原(PCNA)的分布及表达。结果：①39例(60.94％)口腔鳞癌组织表达VEGF，42例(65.63％)表达iNOS；45例(70.31％)表达eNOS；②VEGF与iNOS的表达具有明显相关性，VEGF与eNOS的表达无明显相关性；③表达VEGF的口腔鳞癌PCNA标记指数(PLI)明显高于不表达VEGF的口腔鳞癌；表达iNOS的口腔鳞癌PLI明显高于不表达iNOS的口腔鳞癌。表达eNOS的口腔鳞癌PLI与不表达eNOS的口腔鳞癌无显著性差异(P＞0.05)。结论：①VEGF与iNOS的表达具有明显相关性，说明iNOS在VEGF的生成和发挥作用过程中起重要作用；②PLI随着VEGF和iNOS表达的增加而增加；提示二者对口腔鳞癌细胞增殖具有促进作用。
Objective: To investigate the correlation between the expression of vascular endothelial growth factor (VEGF), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) and the proliferation of cancer cells in oral squamous cell carcinoma. Methods: Immunohistochemistry was used to detect the distribution and expression of VEGF, iNOS, eNOS and proliferating cell nuclear antigen (PCNA) in 64 cases of OSCC. Results: ① 39 cases (60.94%) of oral squamous cell carcinoma expressed VEGF, 42 cases (65.63%) expressed iNOS and 45 cases (70.31%) expressed eNOS. ② The expression of VEGF and iNOS was significantly correlated with the expression of VEGF and eNOS The expression of VEGF in oral squamous cell carcinoma PCNA labeling index (PLI) was significantly higher than those who did not express VEGF in oral squamous cell carcinoma; iNOS expression of oral squamous cell carcinoma was significantly higher than that of non-oral iNOS squamous cell carcinoma. There was no significant difference in the expression of eNOS between oral squamous cell carcinoma and non-eNOS oral squamous cell carcinoma (P> 0.05). CONCLUSION: ①The expression of VEGF and iNOS is significantly correlated, indicating that iNOS plays an important role in the generation and function of VEGF. ②PLI increases with the increase of VEGF and iNOS expression, suggesting that they both promote the proliferation of oral squamous cell carcinoma effect.