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目的:本文首次建立了用1-苯基-3-甲基-5-吡唑啉酮(PMP)作为柱前衍生化试剂对3-O-甲基-葡萄糖(3-OMG)进行衍生化的方法,并应用此方法对大鼠血浆中的3-OMG进行分析。方法:70℃水浴时,3-OMG在0.4 mol/L的NaOH条件下和PMP进行反应。衍生物采用反相高效液相色谱法进行分析,紫外检测波长为245 nm。结果:线性系数在0.991以上,最低定量限0.0078 mg/mL。回收率在98%～105%之间,且日间与日内变异不高于12%。本方法成功应用于糖尿病大鼠血浆中3-OMG的分析。结论:本方法简便、高效,适合对正常和糖尿病大鼠的血浆3-OMG进行分析。
OBJECTIVE: To establish a new method for the derivatization of 3-O-methyl-glucose (3-OMG) with 1-phenyl-3-methyl-5-pyrazolone (PMP) Method and apply this method to analyze 3-OMG in rat plasma. Method: 3-OMG was reacted with PMP under 0.4 mol / L NaOH at 70 ℃. Derivatives were analyzed by reversed-phase high performance liquid chromatography with UV detection at 245 nm. Results: The linearity coefficient was above 0.991 and the lowest limit of quantification was 0.0078 mg / mL. The recoveries ranged from 98% to 105% with a variation of less than 12% during the day and the day. The method was successfully applied to the analysis of 3-OMG in plasma of diabetic rats. Conclusion: This method is simple, efficient and suitable for the analysis of plasma 3-OMG in normal and diabetic rats.