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我们提取纯化得到一种重均分子量为2.03×105、单糖为葡萄糖的香菇多糖级分(L-2)后,评价了香菇多糖L-2对荷S-180小鼠细胞免疫的影响。连续10d通过腹腔注射给予小鼠不同剂量的香菇多糖L-2(10mg/kg体重),测定了正常小鼠与荷S-180小鼠的血清中肿瘤坏死因子(TNF-α)和白介素2(IL-2)的含量,以及香菇多糖L-2对TNF-α和IL-2的mRNA表达的影响。与对照组小鼠相比,血清中TNF-α含量有显著增加,而血清中IL-2含量没有变化,与此同时,香菇多糖L-2可以上调TNF-α的mRNA的表达。结果揭示多糖L-2有增加TNF-α含量的作用,多糖增强免疫力与TNF-α含量增加以及在转录水平上调TNF-αmRNA的表达有关。
We extracted and purified a lentinan fraction (L-2) with a weight average molecular weight of 2.03 × 10 5 and glucose as monomer, and evaluated the effect of lentinan on cellular immunity of S-180 mice. The mice were given intraperitoneal injection of different doses of lentinan L-2 (10 mg / kg body weight) for 10 days. The levels of tumor necrosis factor (TNF-α) and interleukin 2 IL-2), and the effect of lentinan L-2 on the mRNA expression of TNF-α and IL-2. Compared with the control group, serum TNF-α content increased significantly, while serum IL-2 content did not change, at the same time, lentinan L-2 can up-regulate TNF-α mRNA expression. The results revealed that polysaccharide L-2 has the effect of increasing the content of TNF-α, and the enhanced immunity of polysaccharide is related to the increase of TNF-α content and the up-regulation of the expression of TNF-αmRNA at the transcription level.