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目的建立高效液相色谱法同时测定食品中胆固醇、维生素A、α-维生素E的含量的方法。方法采用2.0 mol/L的KOH乙醇溶液对样品直接皂化,石油醚萃取后上机测定,通过实验确定了样品前处理及测定的最佳条件。结果高效液相色谱法能够满足食品中3种食品强化剂同时测定的要求;在0~200μmg/L的线性范围内,r值均大于0.998,胆固醇、维生素A和α-维生素E的回收率均在85.27%~101.44%之间,相对标准偏差均小于5%。结论该新建方法可适用于大多数食品中胆固醇、维生素A、α-维生素E的测定。
Objective To establish a method for the simultaneous determination of cholesterol, vitamin A and α-vitamin E in food by high performance liquid chromatography. Methods The samples were directly saponified with 2.0 mol / L KOH ethanol solution and extracted with petroleum ether. The optimum conditions for sample preparation and determination were determined by experiments. Results High performance liquid chromatography (HPLC) was able to meet the simultaneous determination of three food fortifiers in food. In the linear range of 0-200 μmg / L, the values of r were all greater than 0.998. The recoveries of cholesterol, vitamin A and α-vitamin E Between 85.27% and 101.44%, the relative standard deviations were less than 5%. Conclusion The new method can be applied to the determination of cholesterol, vitamin A and α-vitamin E in most foods.