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为了探讨灌浆结实期高温对水稻籽粒储藏物质以及储藏物质代谢相关基因的影响。我们利用人工气候室设置高温(最高温度35℃/最低温度28℃)和常温(最高温度28℃/最低温度20℃)2个温度处理灌浆结实期的粳稻品种Dongjin,检测胚乳中储藏物质以及储藏物质代谢相关基因的表达。结果表明,高温处理后水稻种子垩白明显增加、重量减小、总淀粉含量和直链淀粉含量下降。高温处理后支链淀粉链长分布也有了明显的改变,DP10～20的短链减少,而DP>20的中长链明显增加。高温处理后淀粉合成相关基因GBSSI和BEIIb的表达量减低。大部分质体转运蛋白和淀粉合成相关基因在高温处理早期表达量增加,而后期下降。高温处理后,多数储藏蛋白基因如GLUB、RAG-1、RM1和PROL14表达量下降。该研究结果将有助于我们了解水稻灌浆期高温影响种子中储藏物质积累的分子机制。
In order to investigate the effects of high temperature during grain filling stage on the grain storage materials and the genes involved in storage metabolism in rice. We set up a japonica rice cultivar Dongjin with high temperature (maximum temperature of 35 ℃ / minimum temperature of 28 ℃) and room temperature (maximum temperature of 28 ℃ / minimum temperature of 20 ℃) in artificial climate chamber to detect storage endosperm storage and storage Metabolism related gene expression. The results showed that after high temperature treatment, the chalkiness of rice seeds increased obviously, the weight decreased, the total starch content and amylose content decreased. The length distribution of amylopectin also changed obviously after high temperature treatment, the short chain of DP10-20 decreased, but the long chain of DP> 20 increased obviously. The expression of GBSSI and BEIIb related to starch synthesis decreased after high temperature treatment. Most plastid transporter and starch synthesis related genes increased in the early stage of high temperature treatment, but later decreased. After high temperature treatment, most of the storage protein genes such as GLUB, RAG-1, RM1 and PROL14 expression decreased. The results of this study will help us to understand the molecular mechanism underlying the accumulation of stored substances in seeds during high temperature during grain filling.