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研究表明,苏云金杆菌(Bacillus thuringiensis)对马尾松毛虫(Dendrolimuspunctatus)幼虫的毒性与苏云金杆菌的不同亚种,同一亚种的不同菌株以及发酵培养基组分有相关性,而在发酵过程中培养基全氮、有机碳、碳氮比例的消长规律与对昆虫的毒性未见一定的相关性。在发酵过程中,同一菌株在同一培养基中的菌数与试虫毒性成正相关;而不同菌株在同一培养基或同一菌株在不同培养基中菌数与对试虫的毒性无关。因此,单纯以活孢子数为指标评价苏云金杆菌不同菌株和不同发酵条件下的杀虫活性是不科学的。
Studies have shown that the toxicity of Bacillus thuringiensis to Dendrolimus punctatus larvae has a correlation with different strains of Bacillus thuringiensis, different strains of the same subspecies, and fermentation medium components. However, in the fermentation process, the medium The total nitrogen, organic carbon, carbon and nitrogen ratio of the growth and decline of insects on the toxicity of no certain relevance. During the fermentation process, the number of bacteria in the same culture medium was positively correlated with the toxicity of test insects. However, the number of bacteria in the same medium or in the same strain of different strains was not related to the toxicity of test insects. Therefore, simply using the number of viable spores as an index to evaluate the insecticidal activity of different strains of Bacillus thuringiensis and different fermentation conditions is unscientific.