论文部分内容阅读
目的探讨Maspin在抗肿瘤转移过程中的作用及微囊化基因修饰细胞治疗恶性肿瘤的可能性和可行性。方法将微囊化Maspin基因修饰的中国仓鼠卵巢上皮细胞(CHO细胞)与乳腺癌细胞Bcap37进行共同培养,观察Bcap37细胞运动功能的变化;观察Bcap37细胞对同质细胞(Bcap37)和异质细胞(人血管内皮细胞ECV204)黏附功能的变化;同时观察被干预后的Bcap37细胞黏附分子CD44v6和E2Cadherin表达的改变。结果经微囊化Maspin基因修饰的CHO细胞处理后的Bcap37细胞,其迁徙功能明显减弱,对Bcap37细胞同质黏附功能明显增强,对ECV204细胞异质黏附功能明显减弱;经微囊化CHO/Maspin细胞处理后,Bcap37细胞的黏附分子E2Cad表达增强,而CD44v6表达有所降低。结论微囊化Maspin基因修饰的CHO细胞对乳腺癌细胞Bcap37的运动和黏附功能有一定影响;Maspin通过抑制肿瘤细胞的运动功能、异质黏附功能和增强同质黏附功能抑制肿瘤细胞的转移;微囊化基因修饰细胞可以用于治疗恶性肿瘤。
Objective To investigate the role of Maspin in anti-tumor metastasis and the possibility and feasibility of microencapsulated genetically modified cells in the treatment of malignant tumors. Methods The microencapsulated Maspin gene-modified Chinese hamster ovary epithelial cells (CHO cells) were co-cultured with breast cancer cell Bcap37 to observe the changes in motor function of Bcap37 cells. Bcap37 cells were observed for homogenous cells (Bcap37) and heterogeneous cells ( The changes of adhesion function of human vascular endothelial cells (ECV204) were observed. At the same time, the expression changes of CD44v6 and E2Cadherin of Bcap37 cells after intervention were observed. Results The migration of Bcap37 cells treated with microencapsulated CHO cells was significantly attenuated, and the function of Bcap37 cell homogenous adhesion was significantly enhanced, and the heterogeneity of ECV204 cells was significantly reduced. Microencapsulated CHO/Maspin After cell treatment, the expression of adhesion molecule E2Cad in Bcap37 cells was enhanced, while the expression of CD44v6 was decreased. Conclusion The microencapsulated CHO cells with Maspin gene have some effects on the function and adhesion of Bcap37 in breast cancer cells. Maspin inhibits the metastasis of tumor cells by inhibiting the motor function, heteromeric adhesion function and enhancing the homogenous adhesion function of tumor cells; Encapsulated genetically modified cells can be used to treat malignant tumors.