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目的检测早期胃癌患者外周血细胞免疫功能变化及意义。方法收集沈阳军区总医院2013年1-12月经手术确诊的胃癌患者58例,其中早期胃癌27例,进展期胃癌31例。采用流式细胞仪方法检测患者外周血辅助性T细胞(CD3+、CD4+、CD8+、CD4+/CD8+)和调节性T细胞(CD4CD25+Treg和CD4+CD25+Foxp3+Treg),并与24例健康志愿者(对照组)相同外周血T细胞亚群表达情况进行比较。结果早期胃癌患者外周血CD3+、CD4+表达浓度及CD4+/CD8+比值显著高于进展期胃癌组,低于健康对照组(P<0.05);CD8+浓度则显著低于进展期胃癌组,高于健康对照组(P<0.05);CD4CD25+Treg及CD4+CD25+Foxp3+Treg表达浓度均低于进展期胃癌组,高于健康对照组(P<0.05)。结论早期胃癌患者外周血细胞免疫功能表达存在差异现象,应进一步研究。
Objective To detect the changes and significance of peripheral blood cell immune function in patients with early gastric cancer. Methods A total of 58 patients with gastric cancer confirmed surgically in the General Hospital of Shenyang Military Region from January to December 2013 were collected. Among them, there were 27 cases of early gastric cancer and 31 cases of advanced gastric cancer. Flow Cytometry was used to detect peripheral blood helper T cells (CD3+, CD4+, CD8+, CD4+/CD8+) and regulatory T cells (CD4CD25+Treg and CD4+CD25+Foxp3+Treg) and 24 healthy volunteers. The same peripheral blood T cell subsets were compared in the control group (control group). Results The expression of CD3+ and CD4+ and CD4+/CD8+ ratio in peripheral blood of patients with early gastric cancer were significantly higher than those in advanced gastric cancer group and lower than those in healthy control group (P<0.05). The concentration of CD8+ was significantly lower than that in advanced gastric cancer group, which was higher than that in healthy controls. The expression of CD4CD25+Treg and CD4+CD25+Foxp3+Treg was significantly lower in the advanced gastric cancer group than in the healthy control group (P<0.05). Conclusion There is a difference in the expression of cellular immune function in peripheral blood of patients with early gastric cancer. It should be further studied.