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目的:建立新型布尼亚病毒IgG抗体ELISA检测方法。方法:用基因工程重组表达的新型布尼亚病毒NP抗原包被酶联板,建立间接ELISA法检测新型布尼亚病毒IgG抗体,并进行特异性和灵敏度评价,健康人群中检测结果计算临界值(均值+3标准差)。检测70例发热伴血小板减少综合征患者恢复血清和69份健康人血清样品。结果:在70份患者血清样品中,检测出新型布尼亚病毒IgG抗体阳性51例,阳性率为72.14%(51/70);69份健康人血清样品中,检测出1份阳性,特异性为98.6%(1/69)。结论:建立的新型布尼亚病毒IgG抗体ELISA检测方法特异性强、灵敏度高,可用于新型布尼亚病毒感染的检测及流行病学调查。
Objective: To establish a new ELISA method for Bunyavirus IgG antibody. Methods: New Bunyavirus NP antigen expressed recombinant plasmid was coated on enzyme-linked immunosorbent assay (ELISA) to establish an indirect ELISA for the detection of new Bunyavirus IgG antibodies. Specificity and sensitivity were evaluated. The cutoff value (Mean +3 standard deviation). Seventy patients with fever and thrombocytopenia syndrome recovered serum and 69 healthy human serum samples. Results: Among the 70 serum samples, 51 positive samples of new Bunyavirus IgG were detected, the positive rate was 72.14% (51/70). Among 69 healthy human serum samples, one positive and one specific 98.6% (1/69). Conclusion: The established ELISA method for the detection of Bunyavirus IgG is highly specific and sensitive and can be used for the detection and epidemiological investigation of new Bunyanovirus infection.