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目的观察褪黑素(MT)对百草枯(PQ)染毒大鼠肾组织中血红素氧合酶-1(HO-1)和诱导型一氧化氮合酶(iNOS)表达的影响,了解MT对PQ中毒肾损伤保护作用。方法SD大鼠随机分空白对照组(A组)、染毒组(B组)和MT治疗组(C组)。B、C组PQ(25mg/kg)、A组等量盐水腹腔注射,15min后C组MT(10mg/kg·d)腹腔注射。观察各组肾组织病理学及HO-1、iNOS表达的变化。结果A组组织结构清晰,B组组织结构清晰度下降,染毒3h即可见充血、水肿及空泡变性等病理变化,1d达峰,其后缓解趋势不明显,C组病理变化明显减轻且缓解趋势明显,A组HO-1多不表达,B组染毒3h呈阳性表达,免疫组织化学评分(IHS)升高(P<0.05),1d达峰,之后减弱。C组较B组表达明显增强,6h～3d差异具有统计学意义(P<0.05);A组iNOS多不表达,B组染毒3h呈阳性表达,1d达峰,其后维持较强表达,C组较B组IHS降低,1d达峰,其后减弱,3d后维持在较低水平,5d时仍有表达(P<0.05)。结论MT对PQ中毒引起的肾损伤有保护作用。
Objective To observe the effect of melatonin (MT) on the expression of heme oxygenase-1 (HO-1) and inducible nitric oxide synthase (iNOS) in paraquat (PQ) PQ poisoning of renal injury protection. Methods SD rats were randomly divided into blank control group (group A), exposure group (group B) and MT treatment group (group C). B, C group PQ (25mg / kg), group A was injected intraperitoneally with equal amount of saline, and group C (10mg / kg · d) was injected intraperitoneally after 15min. The changes of renal histopathology and expression of HO-1 and iNOS in each group were observed. Results The histological structure of group A was clear and the clarity of group B was decreased. Pathological changes such as hyperemia, edema and vacuolar degeneration were observed at 3h after exposure, reaching a peak on day 1, and then the remission was not obvious. The pathological changes of group C were relieved and alleviated The trend was obvious. In group A, the expression of HO-1 was not observed. The expression of HO-1 in group B was positive at 3h, the level of IHS was increased (P <0.05), and reached the peak at 1d. The expression of iNOS in group A was more than that in group B, and the expression of iNOS in group A was more than that in group B (P <0.05) The IHS in group C was lower than that in group B, and reached a peak on day 1, then weakened. After 3 days, the level of IHS was kept at a low level, and remained at 5 days (P <0.05). Conclusion MT has a protective effect on renal injury caused by PQ poisoning.