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目的:观察结肠康对恶唑酮(Oxazolone,OXZ)诱导小鼠溃疡性结肠炎(Ulcerative colitis,UC)炎症反应的抑制作用。方法:小鼠颈背部3%恶唑酮致敏2天,第6天用1.5%恶唑酮灌肠,灌肠当日开始灌胃不同剂量结肠康。灌胃3天后,断头处死小鼠,酶联免疫吸附法(ELISA)测定结肠组织匀浆液中炎性因子IL-4、TNF-α、IL-1β和IL-10浓度。柳氮磺胺吡啶作为阳性对照药。结果:与空白对照组相比模型组IL-4浓度降低64.64%,TNF-α、IL-1β和IL-10浓度增加121.03%、73.04%和213.13%。结肠康6.4、12.8和25.6g/kg组能显著增高IL-4的浓度,降低TNF-α、IL-1β和IL-10浓度,实验结果提示结肠康0.32g/ml、0.64 g/ml和1.28g/ml呈剂量依赖性改善结肠炎症反应细胞因子浓度,结肠康1.28 g/ml剂量组治疗效果最好与阳性对照药SASP无显著差别。结论:结肠康能抑制OXZ诱导小鼠溃疡性结肠炎炎症反应,对UC具有一定得治疗作用。
Objective: To observe the inhibitory effect of Zu Kang on the inflammation of ulcerative colitis (UC) induced by oxazolone (OXZ) in mice. Methods: The mice were sensitized with 3% oxazolone on the back of the neck for 2 days. On the 6th day, 1.5% oxazolone enema was given. Three days after gavage, the mice were sacrificed and the concentrations of inflammatory cytokines IL-4, TNF-α, IL-1β and IL-10 in colonic tissue homogenate were determined by enzyme-linked immunosorbent assay (ELISA) Sulfasalazine as a positive control. Results: Compared with the blank control group, the concentration of IL-4 in model group decreased by 64.64% and the concentrations of TNF-α, IL-1β and IL-10 increased by 121.03%, 73.04% and 213.13%, respectively. The results showed that there were no significant differences between the two groups (P> 0.05) .CONCLUSION 6.4, 12.8 and 25.6 g / kg of KLH can significantly increase the concentration of IL-4 and decrease the concentrations of TNF-α, IL-1β and IL- g / ml in a dose-dependent manner to improve colonic inflammatory cytokines concentration, colon Kang 1.28 g / ml dose group the best treatment with the positive control drug SASP no significant difference. Conclusion: UCK can inhibit the inflammation induced by OXZ in mice with ulcerative colitis, which has a certain therapeutic effect on UC.