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记录单个细胞活动电位目前采用的是金属微电极和玻璃微电极。由于金属微电极茎段较粗,在某些研究中,对组织损伤较大,使用玻璃微电极较好。我们根据玻璃微电极长度,划分微电极为短茎微电极(30～60mm)和长茎微电极(60mm以上)。对于短茎微电极的制备方法已趋成熟,但对于长茎微电极,特别是严格控制长度的长微电极的制备,目前还面临着许多技术困难,现将我们在制备过程中的一些体会和改进提出来,供研讨。
Current single cell recording potentials are used for metal microelectrodes and glass microelectrodes. Due to the thicker stem section of the metal microelectrode, in some studies, the damage to the tissue is larger and the glass microelectrode is better. According to the length of the glass microelectrode, we divided the microelectrodes into short stem microelectrodes (30 ~ 60mm) and long stem microelectrodes (60mm and above). The preparation method of short-stem microelectrodes has matured, but there are still many technical difficulties for the preparation of long-stem microelectrodes, especially the long-length micro-electrodes. Now we have some experience in the preparation process and Improvement put forward for discussion.