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该文旨在研究长非编码RNA Z38对BT549细胞中多能因子表达的影响。通过流式细胞术和实时荧光定量PCR(Real-time quantitative polymerase chain reaction,RT-q PCR)进行Z38与CD44+CD24–/low亚群细胞相关性分析,并利用慢病毒Z38-sh RNA感染BT549细胞以干扰Z38表达,通过RT-qPCR和蛋白质印迹实验检测多能因子表达水平。结果显示,Z38表达水平与CD44+CD24–/low亚群细胞比例存在差异性,以BT549细胞Z38表达水平和CD44+CD24–/low比例相对较高,干扰BT549细胞Z38表达水平后,CD44+CD24–/low比例下降,NANOG和SOX2 m RNA和蛋白质水平皆被下调,而OCT4 m RNA和蛋白质水平无明显变化。干扰Z38影响BT549多能因子的表达,尤其可下调与乳腺癌干细胞分化密切相关的NANOG和SOX2基因表达,提示它可能与乳腺癌干细胞的分化有关。
The aim of this study was to investigate the effect of long non-coding RNA Z38 on pluripotency factor expression in BT549 cells. Z38 and CD44 + CD24- / low subpopulations were analyzed by flow cytometry and real-time quantitative polymerase chain reaction (RT-q PCR), and the lentivirus Z38-sh RNA was used to infect BT549 The cells interfered with Z38 expression, and pluripotent factor expression levels were detected by RT-qPCR and Western blotting. The results showed that the expression of Z38 was significantly different from that of CD44 + CD24- / low subpopulation. The proportion of Z38 in BT549 cells and the proportion of CD44 + CD24- / low in BT549 cells were relatively high, which interfered with the expression of Z38 in BT549 cells. - / low ratio decreased, NANOG and SOX2 m RNA and protein levels were down-regulated, while OCT4 m RNA and protein levels did not change significantly. Interfering Z38 affected the expression of pluripotency factor 5 (BT549), especially down-regulated the expression of NANOG and SOX2, which is closely related to the differentiation of breast cancer stem cells, indicating that it may be related to the differentiation of breast cancer stem cells.