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目的探讨人巨细胞病毒(human cytomegalovirus,HCMV)感染对宿主细胞增殖及凋亡的影响。方法用HCMV AD169感染人胚肺成纤维细胞(human embryonic lung fibroblast cells,HEL),倒置显微镜观察不同感染时间细胞病变,采用MTT法检测HCMV感染对HEL细胞增殖的抑制作用,同时采用流式细胞术检测HCMV感染细胞对照组细胞凋亡指数。结果在HCMV感染48h内,细胞增殖及凋亡指数与细胞对照组无明显差异。自HCMV感染72h后,细胞增殖明显受抑制,凋亡指数明显增高,细胞病变逐渐加重。结论在HCMV感染早期,对宿主细胞的增殖及凋亡影响不明显,在感染后期,HCMV通过抑制宿主细胞增殖,加重细胞病变,促进感染细胞的凋亡而发挥致病作用。
Objective To investigate the effects of human cytomegalovirus (HCMV) infection on the proliferation and apoptosis of host cells. Methods HCMV AD169 was used to infect human embryonic lung fibroblasts (HEL). Inverted microscope was used to observe the cytopathic effect at different times of infection. MTT assay was used to detect the inhibitory effect of HCMV infection on the proliferation of HEL cells. Flow cytometry The apoptosis index of HCMV infected cells in control group was detected. Results Within 48 hours after HCMV infection, there was no significant difference in cell proliferation and apoptosis index compared with the cell control group. 72h after HCMV infection, cell proliferation was significantly inhibited, apoptotic index was significantly increased, and cytopathic gradually increased. Conclusion HCMV infection has no obvious effect on the proliferation and apoptosis of host cells during the early stage of HCMV infection. HCMV plays a pathogenic role at the late stage of infection by inhibiting the proliferation of host cells, exacerbating cytopathic effect and promoting the apoptosis of infected cells.