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对神经细胞在电压钳制方式下进行膜片钳记录受到胞体和突起空间钳位不一致的限制。本文尝试用突起切断方法区分胞体与突起的电压门控型钙电流，并据此对ＭＰＰ＋引起的钙电流变化进行分析。实验结果表明，突起上的电压门控钙通道的最大激活电压约为＋３０ｍＶ，而胞体的则为－１０～１０ｍＶ左右；ＭＰＰ＋损伤多巴胺能神经细胞后可导致电压门控型钙电流的抑制，这一抑制的Ｉ－Ｖ特性与突起钙电流Ｉ－Ｖ非常相近。由此提示，ＭＰＰ＋对于多巴胺能神经细胞的损伤更集中于其突起纤维。
Patch-clamp recording of nerve cells under voltage clamping is limited by inconsistencies in cell and bump space clamps. In this paper, we try to distinguish the voltage-gated Ca2 + currents of cell bodies and protuberances by using the protrusion-cutting method and analyze the changes of calcium current caused by MPP +. The experimental results show that the maximum activation voltage of the voltage-gated calcium channel on the protrusion is about +30 mV and the cell body is about -10-10 mV; MPP + can damage the voltage-gated calcium current after the dopaminergic neurons damage, which The inhibitory I-V property is very similar to the protruding calcium current I-V. It is suggested that MPP + damage to dopaminergic neurons is more focused on their protruding fibers.